Laboratory tomographic microscopy with compact plasma based extreme ultraviolet and soft x-ray sources
Microscopy using the Soft X-ray (SXR) and Extreme Ultraviolet (EUV) radiation is a very promising technique in micro- and nano-structure imaging. It can provide quality images of cells and its organelles and information of the chemistry of proteins and other bio-molecules. This is possible because of the so called ‘water window’ interaction of light with wavelength from 2.3 – 4.4 nm.
The typical SXR and EUV radiation sources for Tomographic Microscopy are Synchrotron Accelerators. These sources are quite expensive and not accessible to everyone in a typical research laboratory. Hence, there is a need for a compact, practical and less expensive source of SXR and EUV. A recently developed Compact SXR EUV source based on Gas-discharge Plasma were developed by Bergmann, K. et al.
Two dimensional images of biological cells have been produced with nanometer resolution. By rotating the sample, it is possible that a tomographic images can be acquired. This is done through an algorithm similar to the principle behind Computed Tomography X-ray machines. Two dimensional projection images can be reconstructed to produce a three dimensional image or tomography image.
Daniel D. Vicario is a Joint Doctorate scholar for Erasmus Mundus EXTATIC PhD program for Experimental Physics. He obtained his BS degree with an outstanding graduate award in Applied Physics major in Instrumentation from the College of Science, University of Santo Tomas (UST) in the Philippines. He finished his M.Sc. degree in Applied Physics major in Medical Physics at The Graduate School, also in UST.
He has taught Physics and Instrumentation courses for seven years in UST and has three years clinical training for Radiation Oncology Medical Physics in UST Hospital. His research interests include Medical Imaging Physics (CT X-ray machines), Radiation Therapy Physics (Linear Accelerator), and Non-Ionizing Radiation Protection (ELF EMF). He is currently working on his research project on Laboratory tomographic microscopy.